PCR

1. Amplification of a segment of DNA located between synthetic oligonucleotide primers

2. Experimentally simple, yet permits amplifications so large that signals can be detected from single DNA molecules

3. Numerous variations available

4. Multiple applications

• Information about PCR from the Promega Corporation, including a nice diagram of the reaction.
• The PCR Jump Site provides a useful compendium of PCR theory, diagrams and protocols.
• A protocol for PCR-mediated site-directed mutagenesis is available by clicking here.

This page was updated August 30, 1998.

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